[Soul Eater]

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Crona (クロナ, Kurona; Literally meaning "Dark One") is the first reoccurring antagonist of Soul Eater. The child of Medusa Gorgon, Crona is a Demon Sword Master whose weapon, Ragnarok, resides permanently within his/her blood. Crona was Medusa's first attempt at creating a Kishin, but instead of turning into a Demon god, Crona instead turned to help Shibusen defeat Medusa and ultimately, the Kishin. Crona is presented with an ambiguous gender throughout the entire series and while there have been hints toward Crona's sex in both directions, nothing definitive has been presented.

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Crona is presented throughout the series as having an ambiguous sex, and although hints are given to identify the gender, nothing has ever stated it exactly. It is important to note that Crona refers to himself/herself with the masculine pronoun boku (僕)— Japanese for "I", which is used mainly by, but not exclusively, males. [2] However, the term is becoming more popular with females and is not unheard of to be used by a girl, especially tomboys. This is seen in other anime such as Souseiseki in Rozen Maiden for example. Also, Medusa always calls crona as "my child", never refering something more specific as "son" or "daughter".

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credit : http://souleater.wikia.com/wiki/Crona